Analysis of the influence of flame sterilization included in sampling operations on shake-flask cultures of microorganisms.

ABSTRACT: Shake-flask cultures of microorganisms involve flame sterilization during sampling, which produces combustion gas with high CO₂ concentrations. The gaseous destination has not been deeply analyzed. Our aim was to investigate the effect of flame sterilization on the headspace of the flask and on the shake-flask culture. In this study, the headspace CO₂ concentration was found to increase during flame sterilization ~0.5-2.0% over 5-20 s empirically using the Circulation Direct Monitoring and Sampling System. This CO₂ accumulation was confirmed theoretically using Computational Fluid Dynamics; it was 9% topically. To evaluate the influence of CO₂ accumulation without interference from other sampling factors, the flask gas phase formed by flame steri

SUBMITTER: Takahashi M 

PROVIDER: S-EPMC7326993 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature