A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode.

ABSTRACT: A uric acid (UA) electrochemical biosensor was constructed using ferrocene (Fc) decorated cuprous oxide (Cu₂O) enhanced electro-active characteristics and covalently immobilized with uricase (UOx) on glassy carbon electrode (GCE). The electrochemical characteristics of the fabricated electrode was analysed by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry (DPV). DPV studies revealed rapid response of fabricated electrode UOx/Fc/Cu₂O/GCE towards UA in a wide concentration range of 0.1-1,000 μM with a sensitivity of 1.900 μA mM^-1 cm^-2 and very low detection limit of 0.0596 μM. A very low magnitude Michaelis-Menten constant (Km) value was evaluated as 34.7351 μM which indicated the chemical attraction o

SUBMITTER: Yan Q 

PROVIDER: S-EPMC7327035 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature