Quantitative kinetics of intracellular singlet oxygen generation using a fluorescence probe.

ABSTRACT: Singlet oxygen (¹O₂) is a type of reactive oxygen species involved in numerous physiological activities. We previously reported that ¹O₂-specific oxidation products are increased in patients with prediabetes, suggesting that measurement of ¹O₂ may be an important indicator of physiological and pathological conditions. The turnover in the generation and quenching of ¹O₂ is extremely rapid during biological activities owing to it high reactivity and short lifetime in solution. However, the dynamic changes in ¹O₂ generation in living cells have not been fully explored. In this study, we investigated whether the kinetics of ¹O₂ generation can be quantified using a

SUBMITTER: Murotomi K 

PROVIDER: S-EPMC7327044 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature