Endoribonuclease YbeY Is Essential for RNA Processing and Virulence in Pseudomonas aeruginosa.

ABSTRACT: Posttranscriptional regulation plays an essential role in the quick adaptation of pathogenic bacteria to host environments, and RNases play key roles in this process by modifying small RNAs and mRNAs. We find that the Pseudomonas aeruginosa endonuclease YbeY is required for rRNA processing and the bacterial virulence in a murine acute pneumonia model. Transcriptomic analyses reveal that knocking out the ybeY gene results in downregulation of oxidative stress response genes, including the catalase genes katA and katB Consistently, the ybeY mutant is more susceptible to H₂O₂ and neutrophil-mediated killing. Overexpression of katA restores the bacterial tolerance to H₂O₂ and neutrophil killing as well as vir

SUBMITTER: Xia Y 

PROVIDER: S-EPMC7327168 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature