Downregulation of long non-coding RNA LINP1 inhibits the malignant progression of esophageal squamous cell carcinoma.

ABSTRACT:

Background

Long noncoding RNA (lncRNA) in non-homologous end joining pathway 1 (LINP1) contributes to tumorigenesis in various cancers. However, little has been known about the role of LINP1 in esophageal squamous cell carcinoma (ESCC).

Methods

LINP1 was selected as the target lncRNA by bioinformatics analysis. The relationship between LINP1 expression and prognosis was analyzed in 122 ESCC patients. LINP1 status was evaluated by fluorescence in situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR) in normal esophageal tissues, ESCC tissues and EC9706 cells. Short hairpin RNA transfection was used to silence LINP1 in EC9706 cells. Clone formation assay, transwell migration assay, flow cytometry, and tumorigenesis experiment were performed to evaluate the maligna

SUBMITTER: Lu T 

PROVIDER: S-EPMC7327344 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature