Single-molecule optical microscopy of protein dynamics and computational analysis of images to determine cell structure development in differentiating <i>Bacillus subtilis</i>.

ABSTRACT: Here we use singe-molecule optical proteomics and computational analysis of live cell bacterial images, using millisecond super-resolved tracking and quantification of fluorescently labelled protein SpoIIE in single live Bacillus subtilis bacteria to understand its crucial role in cell development. Asymmetric cell division during sporulation in Bacillus subtilis presents a model system for studying cell development. SpoIIE is a key integral membrane protein phosphatase that couples morphological development to differential gene expression. However, the basic mechanisms behind its operation remain unclear due to limitations of traditional tools and technologies. We instead used advanced single-molecule imaging of fluorescently tagged SpoIIE in real time on living cells to reve

SUBMITTER: Wollman AJM 

PROVIDER: S-EPMC7327415 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature