Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.

ABSTRACT: Modular polyketide synthases (PKSs) are a multidomain megasynthase class of biosynthetic enzymes that have great promise for the development of new compounds, from new pharmaceuticals to high value commodity and specialty chemicals. Their colinear biosynthetic logic has been viewed as a promising platform for synthetic biology for decades. Due to this colinearity, domain swapping has long been used as a strategy to introduce molecular diversity. However, domain swapping often fails because it perturbs critical protein-protein interactions within the PKS. With our increased level of structural elucidation of PKSs, using judicious targeted mutations of individual residues is a more precise way to introduce molecular diversity with less potential for global disruption of the protein architect

SUBMITTER: Drufva EE 

PROVIDER: S-EPMC7327777 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature