Enrichment of the airway microbiome in people living with HIV with potential pathogenic bacteria despite antiretroviral therapy.

ABSTRACT:

Background

Long-term antiretroviral therapy (ART) enables people living with HIV (PLW-HIV) to be healthier and live longer; though they remain at greater risk of pneumonia and chronic lung disease than the general population. Lung microbial dysbiosis has been shown to contribute to respiratory disease.

Methods

16S-rRNA gene sequencing on the Miseq-platform and qPCR for typical respiratory pathogens were performed on sputum samples collected from 64 PLW-HIV (median blood CD4 count 676 cells/μL) and 38 HIV-negative participants.

Finding

Richness and α-diversity as well as the relative-abundance (RA) of the major taxa (RA>1%) were similar between both groups. In unweighted-Unifrac ß-diversity, the samples from PLW-HIV showed greater diversity, in contrast to the HIV nega

SUBMITTER: Rofael SAD 

PROVIDER: S-EPMC7327893 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature