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Posttranslational modification of Elongation Factor P from Staphylococcus aureus.

ABSTRACT: Antibiotic-resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF-P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF-P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF-P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF-P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5-aminopentanol modification of S. aureus EF-P.

SUBMITTER: Golubev A

PROVIDER: S-EPMC7327921 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Posttranslational modification of Elongation Factor P from Staphylococcus aureus.

Golubev Alexander A Negroni Luc L Krasnovid Filipp F Validov Shamil S Yusupova Gulnara G Yusupov Marat M Usachev Konstantin K

FEBS open bio 20200616 7

Antibiotic-resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF-P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF-P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF-P posttranslational modificati ...[more]

PMID: 32436337

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