Neurogranin regulates eNOS function and endothelial activation.

ABSTRACT: Endothelial nitric oxide (NO) is a critical mediator of vascular function and vascular remodeling. NO is produced by endothelial nitric oxide synthase (eNOS), which is activated by calcium (Ca²⁺)-dependent and Ca²⁺-independent pathways. Here, we report that neurogranin (Ng), which regulates Ca²⁺-calmodulin (CaM) signaling in the brain, is uniquely expressed in endothelial cells (EC) of human and mouse vasculature, and is also required for eNOS regulation. To test the role of Ng in eNOS activation, Ng knockdown in human aortic endothelial cells (HAEC) was performed using Ng SiRNA along with Ng knockout (Ng ^-/-) in mice. Depletion of Ng expression decreased eNOS activity in HAEC and NO production in mice. We show that Ng expression was decreased by

SUBMITTER: Cheriyan VT 

PROVIDER: S-EPMC7327963 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature