Hat1-Dependent Lysine Acetylation Targets Diverse Cellular Functions.

ABSTRACT: Lysine acetylation has emerged as one of the most important post-translational modifications, regulating different biological processes. However, its regulation by lysine acetyltransferases is still unclear in most cases. Hat1 is a lysine acetyltransferase originally identified based on its ability to acetylate histones. Using an unbiased proteomics approach, we have determined how loss of Hat1 affects the mammalian acetylome. Hat1^+/+ and Hat1^-/- mouse embryonic fibroblast cell lines were grown in both glucose- and galactose-containing media, as Hat1 is required for growth on galactose, and Hat1^-/- cells exhibit defects in mitochondrial function. Following trypsin digestion of whole cell extracts, acetylated peptides were enriched by acetyllysine affinity p

SUBMITTER: Agudelo Garcia PA 

PROVIDER: S-EPMC7328124 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature