Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro.

ABSTRACT: Satellite cells (SC) are muscle stem cells that can regenerate adult muscles upon injury. Most SC originate from PAX7⁺ myogenic precursors set aside during development. Although myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we report the generation of human induced pluripotent stem cell (iPSC) reporter lines in which fluorescent proteins have been introduced into the PAX7 and MYOG loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPSC-derived PAX7⁺ myogenic precursors. We show that the PAX7⁺ cells generated in culture can produce myofibers and self-renew in vitro and in vivo Together, we demonstrate that cells exhibiting charact

SUBMITTER: Al Tanoury Z 

PROVIDER: S-EPMC7328153 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature