Evolving sequence mutations in the Middle East Respiratory Syndrome Coronavirus (MERS-CoV).

ABSTRACT:

Background

Middle East respiratory syndrome coronavirus (MERS-CoV) has continued to cause sporadic outbreaks of severe respiratory tract infection over the last 8 years.

Methods

Complete genome sequencing using next-generation sequencing was performed for MERS-CoV isolates from cases that occurred in Riyadh between 2015 and 2019. Phylogenetic analysis and molecular mutational analysis were carried out to investigate disease severity.

Results

A total of eight MERS-CoV isolates were subjected to complete genome sequencing. Phylogenetic analysis resulted in the assembly of 7/8 sequences within lineage 3 and one sequence within lineage 4 showing complex genomic recombination. The isolates contained a variety of unique amino acid substitutions in ORF1ab (41), the N protein

SUBMITTER: AlBalwi MA 

PROVIDER: S-EPMC7328614 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature