Exploring the Interaction of Cobalt Oxide Nanoparticles with Albumin, Leukemia Cancer Cells and Pathogenic Bacteria by Multispectroscopic, Docking, Cellular and Antibacterial Approaches.

ABSTRACT:

Aim

The interaction of NPs with biological systems may reveal useful details about their pharmacodynamic, anticancer and antibacterial effects.

Methods

Herein, the interaction of as-synthesized Co₃O₄ NPs with HSA was explored by different kinds of fluorescence and CD spectroscopic methods, as well as molecular docking studies. Also, the anticancer effect of Co₃O₄ NPs against leukemia K562 cells was investigated by MTT, LDH, caspase, real-time PCR, ROS, cell cycle, and apoptosis assays. Afterwards, the antibacterial effects of Co₃O₄ NPs against three pathogenic bacteria were disclosed by antibacterial assays.

Results

Different characterization methods such as TEM, DLS, zeta potential and XRD studies prov

SUBMITTER: Arsalan N 

PROVIDER: S-EPMC7328876 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature