HiCoP, a simple and robust method for detecting interactions of regulatory regions.

ABSTRACT:

Background

Chromatin physical interactions provide essential information for understanding the regulation of cis-elements like enhancers, promoters, and insulators in cell development and differentiation. The Hi-C assay is a technique detecting chromatin structures of the whole genome, but not sensitive to interactions of regulatory elements. Several methods, like HiChIP, DNase-C, and OCEAN-C, have been developed for enriching interactions of regulatory regions, but all of them have some shortcomings. New simple, efficient, and robust methods are still in need for detecting interactions of regulatory regions.

Results

We developed a new, simple, and robust assay called CoP (Column Purified chromatin) for profiling of open chromatin regions by directly purifying fragmentized c

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7329539 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature