MicroRNA let-7b inhibits cell proliferation via upregulation of p21 in hepatocellular carcinoma.

ABSTRACT:

Background

Hepatocellular carcinoma (HCC) is one of the most malignant tumor types and has a high incidence and mortality. Many miRNAs play important roles in the development of HCC. Identification of these miRNAs and their targets is increasingly urgent for a better understandingof miRNA function in both physiological and pathological contexts. Many studies have shown that the expression of let-7 is often downregulated in the process of tumorigenesis, suggesting that let-7 may participate in this process as an oncogene.

Methods

Immunochemistry staining was used to observe the expression of let-7b in HCC tissues. A CCK-8 assay was employed to detect the role of let-7b in the proliferation of HCC cells. The cell cycle of HCC cells was examined by flow cytometry. BALB/c nu/nu

SUBMITTER: Hui L 

PROVIDER: S-EPMC7329548 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature