Primary resistance to first-generation EGFR-TKIs induced by MDM2 amplification in NSCLC.

ABSTRACT:

Introduction

Targeted therapy for NSCLC is rapidly evolving. EGFR-TKIs benefit NSCLC patients with sensitive EGFR mutations and significantly prolong survival. However, 20-30% of patients demonstrate primary resistance to EGFR-TKIs, which leads to the failure of EGFR-TKI treatment. The mechanisms of primary resistance to EGFR-TKIs require further study.

Methods

Targeted sequencing was used for the detection of genomic alterations among patients in our center. Regular cell culture and transfection with plasmids were used to establish NSCLC cell lines over-expressing MDM2 and vector control. We used the MTT assays to calculate the inhibition rate after exposure to erlotinib. Available datasets were used to determine the role of MDM2 in the prognosis of NSCLC.

Results

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SUBMITTER: Sun D 

PROVIDER: S-EPMC7329552 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature