MiR-10a overexpression aggravates renal ischemia-reperfusion injury associated with decreased PIK3CA expression.

ABSTRACT: BACKGROUND:To investigate the effect of miR-10a on renal tissues with ischemia reperfusion (I/R) injury in rats and to explore the underlying mechanisms of the effect of miR-10a on hypoxia-reoxygenation in HK-2 cells. METHODS:MiR-10a level was measured in the renal tissues of rats with I/R rats using RT-PCR. In order to research the role of miR-10a in renal tissues, an miR-10 agonist and an miR-10a antagonist were used to treat I/R-injured rats. Levels of serum creatinine and blood urea nitrogen, renal histopathology, and levels of cell apoptosis were analyzed separately in renal tissues from the rats. Phosphatidylinositol 3-kinase (PI3K)/Akt pathway related proteins were measured by Western blotting. In addition, HK-2 cells were cultured in order to study the mechanism of action of miR-10a in the hypoxia-reoxygenation model being studied. Finally, the dual luciferase reporter gene assay was used to confirm that the PI3K p100 catalytic subunit ? (PIK3CA) gene was targeted by miR-10a. RESULTS:After renal I/R injury in rats, miR-10a expression increased significantly (p?

SUBMITTER: Xu D 

PROVIDER: S-EPMC7329557 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature