Brief ex vivo Fas-ligand incubation attenuates GvHD without compromising stem cell graft performance.

ABSTRACT: Graft versus host disease (GvHD) remains a limiting factor for successful hematopoietic stem cell transplantation (HSCT). T cells and antigen-presenting cells (APCs) are major components of the hematopoietic G-CSF mobilized peripheral blood cell (MPBC) graft. Here we show that a short incubation (2 h) of MPBCs with hexameric Fas ligand (FasL) selectively induces apoptosis of specific donor T cell subsets and APCs but not of CD34⁺ cells. FasL treatment preferentially induces apoptosis in mature T cell subsets which express high levels of Fas (CD95), such as T stem cell memory, T central memory, and T effector memory cells, as well as T_H1 and T_H17 cells. Anti-CD3/CD28 stimulated T cells derived from FasL-treated-MPBCs express lower levels of CD25 and secrete

SUBMITTER: Levy-Barazany H 

PROVIDER: S-EPMC7329633 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature