Estrogenic activity of food contact materials-evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates.

ABSTRACT: Food contact materials (FCM) may contain complex mixtures of estrogenic chemicals. A yeast estrogen screen performed on high performance thin-layer chromatography plates (planar-YES, P-YES) is promising for analysis of such mixtures, as it could allow for better elucidation of effects compared with established methods in microtiter plates. However, the P-YES has not been directly compared with established methods. We compared the performance of a microtiter plate YES (lyticase-YES, L-YES) to P-YES on silica gel HPTLC plates using 17β-estradiol (E2), 20 chemicals representative of migrants from plastic FCM, and three migrates of coated metal food cans. Effective doses (ED₁₀, ED₅₀) and estradiol equivalencies were calculated for each chemical. Thirteen chemicals had cal

SUBMITTER: Bergmann AJ 

PROVIDER: S-EPMC7329773 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature