Proteus: An algorithm for proposing stabilizing mutation pairs based on interactions observed in known protein 3D structures.

ABSTRACT:

Background

Protein engineering has many applications for industry, such as the development of new drugs, vaccines, treatment therapies, food, and biofuel production. A common way to engineer a protein is to perform mutations in functionally essential residues to optimize their function. However, the discovery of beneficial mutations for proteins is a complex task, with a time-consuming and high cost for experimental validation. Hence, computational approaches have been used to propose new insights for experiments narrowing the search space and reducing the costs.

Results

In this study, we developed Proteus (an acronym for Protein Engineering Supporter), a new algorithm for proposing mutation pairs in a target 3D structure. These suggestions are based on contacts observed in

SUBMITTER: Barroso JRMS 

PROVIDER: S-EPMC7330979 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature