Neural circuit analysis using a novel intersectional split intein-mediated split-Cre recombinase system.

ABSTRACT: The defining features of a neuron are its functional and anatomical connections with thousands of other neurons in the brain. Together, these neurons form functional networks that direct animal behavior. Current approaches that allow the interrogation of specific populations of neurons and neural circuits rely heavily on targeting their gene expression profiles or connectivity. However, these approaches are often unable to delineate specific neuronal populations. Here, we developed a novel intersectional split intein-mediated split-Cre recombinase system that can selectively label specific types of neurons based on their gene expression profiles and structural connectivity. We developed this system by splitting Cre recombinase into two fragments with evolved split inteins and subsequently

SUBMITTER: Khoo ATT 

PROVIDER: S-EPMC7331137 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature