Using osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803.

ABSTRACT:

Background

Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO₂ by photosynthetic cyanobacteria, has become increasingly appealing. However, previous work aiming to achieve mannitol production in the marine Synechococcus sp. PCC7002 via heterologous expression of mannitol-1-phosphate-5-dehydrogenase (mtlD) and mannitol-1-phosphatase (m1p, in short: a 'mannitol cassette'), proved to be genetically unstable. In this study, we aim to overcome this genetic instability by conceiving a strategy to stabilize mannitol production using Synechocystis sp. PCC6803 as a model cyanobacterium.

Results

Here,

SUBMITTER: Wu W 

PROVIDER: S-EPMC7331161 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature