Genome-wide analyses of chromatin interactions after the loss of Pol I, Pol II, and Pol III.

ABSTRACT:

Background

The relationship between transcription and the 3D chromatin structure is debated. Multiple studies have shown that transcription affects global Cohesin binding and 3D genome structures. However, several other studies have indicated that inhibited transcription does not alter chromatin conformations.

Results

We provide the most comprehensive evidence to date to demonstrate that transcription plays a relatively modest role in organizing the local, small-scale chromatin structures in mammalian cells. We show degraded Pol I, Pol II, and Pol III proteins in mESCs cause few or no changes in large-scale 3D chromatin structures, selected RNA polymerases with a high abundance of binding sites or active promoter-associated interactions appear to be relatively more affected

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC7331254 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature