Epidemiology and genetic diversity of circulating dengue viruses in Medellin, Colombia: a fever surveillance study.

ABSTRACT:

Background

Dengue fever is a major public health problem in Colombia. A fever surveillance study was conducted for evaluation of the clinical, epidemiological, and molecular patterns of dengue, prior to Chikungunya and Zika epidemics.

Methods

In November 2011-February 2014, a passive facility-based surveillance was implemented in Santa Cruz Hospital, Medellin, and enrolled eligible febrile patients between 1 and 65 years-of-age. Acute and convalescent blood samples were collected 10-21 days apart and tested for dengue using IgM/IgG ELISA. RNA was extracted for serotyping using RT-PCR on acute samples and genotyping was performed by sequencing.

Results

Among 537 febrile patients enrolled during the study period, 29% (n = 155) were identified to be dengue-positive. Only

SUBMITTER: Lim JK 

PROVIDER: S-EPMC7331258 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature