Web portal for analytical validation of MRM-MS assay abided with integrative multinational guidelines.

ABSTRACT: Multiple reaction monitoring-mass spectrometry became a mainstream method for quantitative proteomics, which made the validation of a method and the analyzed data important. In this portal for validation of the MRM-MS assay, we developed a website that automatically evaluates uploaded MRM-MS data, based on biomarker assay guidelines from the European Medicines Agency, the US Food & Drug Administration, and the Korea Food & Drug Administration. The portal reads a Skyline output file and produces the following results-calibration curve, specificity, sensitivity, carryover, precision, recovery, matrix effect, recovery, dilution integrity, stability, and QC-according to the standards of each independent agency. The final tables and figures that pertain to the 11 evaluation categories are displ

SUBMITTER: Kim J 

PROVIDER: S-EPMC7331696 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature