Decipher the complexity of cis-regulatory regions by a modified Cas9.

ABSTRACT: BACKGROUND:Understanding complex mechanisms of human transcriptional regulation remains a major challenge. Classical reporter studies already enabled the discovery of cis-regulatory elements within the non-coding DNA; however, the influence of genomic context and potential interactions are still largely unknown. Using a modified Cas9 activation complex we explore the complexity of renin transcription in its native genomic context. METHODS:With the help of genomic editing, we stably tagged the native renin on chromosome 1 with the firefly luciferase and stably integrated a programmable modified Cas9 based trans-activation complex (SAM-complex) by lentiviral transduction into human cells. By delivering five specific guide-RNA homologous to specific promoter regions of renin we were able to g

SUBMITTER: Kirchner S 

PROVIDER: S-EPMC7332081 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature