Histological and quantitative polymerase chain reaction-based analysis of Buruli ulcer using mapping biopsy method.

ABSTRACT:

Background

In Japan, Buruli ulcer cases are often advanced, requiring surgical treatment. However, extensive debridement is often difficult because of cosmetic and functional sequelae. Moreover, the lesions are complicated and composed of edematous erythema, necrotic ulcer, and erythematous skin lesions caused by a paradoxical reaction, which also make it difficult to perform adequate debridement.

Methodology/principal findings

We performed quantitative polymerase chain reaction (PCR) analysis for IS2404 using 29 samples taken from mapping biopsy. We evaluated the relationship among mycobacterial burden, histopathological findings, and clinical outcomes using 83 tissue samples taken from mapping biopsy and debrided Buruli ulcer. On quantitative PCR, the Cp values of IS2404 a

SUBMITTER: Takahashi T 

PROVIDER: S-EPMC7332088 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature