Assessing predictors for new post translational modification sites: A case study on hydroxylation.

ABSTRACT: Post-translational modification (PTM) sites have become popular for predictor development. However, with the exception of phosphorylation and a handful of other examples, PTMs suffer from a limited number of available training examples and sparsity in protein sequences. Here, proline hydroxylation is taken as an example to compare different methods and evaluate their performance on new experimentally determined sites. As a guide for effective experimental design, predictors require both high specificity and sensitivity. However, the self-reported performance may often not be indicative of prediction quality and detection of new sites is not guaranteed. We have benchmarked seven published hydroxylation site predictors on two newly constructed independent datasets. The self-reported performa

SUBMITTER: Piovesan D 

PROVIDER: S-EPMC7332089 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature