Massively multiplexed nucleic acid detection with Cas13.

ABSTRACT: The great majority of globally circulating pathogens go undetected, undermining patient care and hindering outbreak preparedness and response. To enable routine surveillance and comprehensive diagnostic applications, there is a need for detection technologies that can scale to test many samples^1-3 while simultaneously testing for many pathogens^4-6. Here, we develop Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (CARMEN), a platform for scalable, multiplexed pathogen detection. In the CARMEN platform, nanolitre droplets containing CRISPR-based nucleic acid detection reagents⁷ self-organize in a microwell array⁸ to pair with droplets of amplified samples, testing each sample against each CRISPR RNA (crRNA) in replicate.

SUBMITTER: Ackerman CM 

PROVIDER: S-EPMC7332423 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature