Comparative proteomics of three Giardia lamblia strains: investigation of antigenic variation in the post-genomic era.

ABSTRACT: Giardia lamblia is a causative agent of persistent diarrhoea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well-characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identified 398 proteins differentially expressed between the GS and both WB isolates and 97 proteins differentially expressed between the two WB isolates. We investigated the expression levels of the predominant variant-specific surface proteins (VSPs) in each clone and matched the previously described major

SUBMITTER: Muller J 

PROVIDER: S-EPMC7332775 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature