The 18S ribosomal RNA m⁶ A methyltransferase Mettl5 is required for normal walking behavior in Drosophila.

ABSTRACT: RNA modifications have recently emerged as an important layer of gene regulation. N6-methyladenosine (m⁶ A) is the most prominent modification on eukaryotic messenger RNA and has also been found on noncoding RNA, including ribosomal and small nuclear RNA. Recently, several m⁶ A methyltransferases were identified, uncovering the specificity of m⁶ A deposition by structurally distinct enzymes. In order to discover additional m⁶ A enzymes, we performed an RNAi screen to deplete annotated orthologs of human methyltransferase-like proteins (METTLs) in Drosophila cells and identified CG9666, the ortholog of human METTL5. We show that CG9666 is required for specific deposition of m⁶ A on 18S ribosomal RNA via direct interaction with the Droso

SUBMITTER: Leismann J 

PROVIDER: S-EPMC7332798 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature