Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish.

ABSTRACT: The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III₂  + IV₂ , I + IV, and I + III₂  + IV₂ . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1^-/- fish displayed altered OXPHOS activity due to the disrupted interaction of com

SUBMITTER: Garcia-Poyatos C 

PROVIDER: S-EPMC7332985 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature