Unveiling protist diversity associated with the Pacific oyster Crassostrea gigas using blocking and excluding primers.

ABSTRACT:

Background

Microbiome of macroorganisms might directly or indirectly influence host development and homeostasis. Many studies focused on the diversity and distribution of prokaryotes within these assemblages, but the eukaryotic microbial compartment remains underexplored so far.

Results

To tackle this issue, we compared blocking and excluding primers to analyze microeukaryotic communities associated with Crassostrea gigas oysters. High-throughput sequencing of 18S rRNA genes variable loops revealed that excluding primers performed better by not amplifying oyster DNA, whereas the blocking primer did not totally prevent host contaminations. However, blocking and excluding primers showed similar pattern of alpha and beta diversities when protist communities were sequenced using

SUBMITTER: Clerissi C 

PROVIDER: S-EPMC7333408 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature