One-step generation of composite soybean plants with transgenic roots by Agrobacterium rhizogenes-mediated transformation.

ABSTRACT:

Background

Agrobacterium rhizogenes-mediated (ARM) transformation is a highly efficient technique for generating composite plants composed of transgenic roots and wild-type shoot, providing a powerful tool for studying root biology. The ARM transformation has been established in many plant species, including soybean. However, traditional transformation of soybean, transformation efficiency is low. Additionally, the hairy roots were induced in a medium, and then the generated composite plants were transplanted into another medium for growth. This two-step operation is not only time-consuming, but aggravates contamination risk in the study of plant-microbe interactions.

Results

Here, we report a one-step ARM transformation method with higher transformation efficiency for gener

SUBMITTER: Fan YL 

PROVIDER: S-EPMC7333419 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature