Genome analysis of peeling archival cytology samples detects driver mutations in lung cancer.

ABSTRACT:

Introductions

When tumor tissue samples are unavailable to search for actionable driver mutations, archival cytology samples can be useful. We investigate whether archival cytology samples can yield reliable genomic information compared to corresponding formalin-fixed paraffin-embedded (FFPE) tumor samples.

Patients and methods

Pretreatment class V archival cytology samples with adequate tumor cells were selected from 172 lung cancer patients. The genomic profiles of the primary lung tumors have been analyzed through whole-exome regions of 53 genes. We compared the genomic profiles based on the oncogenicity and variant allele frequency (VAF) between the archival cytology and the corresponding primary tumors. We also analyzed the genomic profiles of serial cytological samples

SUBMITTER: Kunimasa K 

PROVIDER: S-EPMC7333826 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature