Improved targeting of an anti-TAG-72 antibody drug conjugate for the treatment of ovarian cancer.

ABSTRACT:

Introduction

Ovarian cancer has only a 17% 5-year survival rate in patients diagnosed with late stage disease. Tumor-associated glycoprotein-72 (TAG72), expressed in 88% of all stages of ovarian cancer, is an excellent candidate for antibody-targeted therapy, as it is not expressed in normal human adult tissues, except in the secretory endometrium.

Methods

Using the clinically relevant anti-TAG72 murine monoclonal antibody CC49, we evaluated antibody drug conjugates (ADCs) incorporating the highly potent, synthetic antimitotic agent monomethylauristatin E (MMAE). MMAE was conjugated to CC49 via reduced disulfides in the hinge region, using three different types of linker chemistry, vinylsulfone (VS-MMAE), bromoacetamido (Br-MMAE), and maleimido (mal-MMAE).

Results

The

SUBMITTER: Minnix M 

PROVIDER: S-EPMC7333846 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature