Harmonizing Cell-Free DNA Collection and Processing Practices through Evidence-Based Guidance.

ABSTRACT: Circulating cell-free DNA (cfDNA) is rapidly transitioning from discovery research to an important tool in clinical decision making. However, the lack of harmonization of preanalytic practices across institutions may compromise the reproducibility of cfDNA-derived data and hamper advancements in cfDNA testing in the clinic. Differences in cellular genomic contamination, cfDNA yield, integrity, and fragment length have been attributed to different collection tube types and anticoagulants, processing delays and temperatures, tube agitation, centrifugation protocols and speeds, plasma storage duration and temperature, the number of freeze-thaw events, and cfDNA extraction and quantification methods, all of which can also ultimately impact subsequent downstream analysis. Thus, there is a press

SUBMITTER: Greytak SR 

PROVIDER: S-EPMC7334102 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature