Endothelial Palmitoylation Cycling Coordinates Vessel Remodeling in Peripheral Artery Disease.

ABSTRACT:

Rationale

Peripheral artery disease, common in metabolic syndrome and diabetes mellitus, responds poorly to medical interventions and is characterized by chronic vessel immaturity leading to lower extremity amputations.

Objective

To define the role of reversible palmitoylation at the endothelium in the maintenance of vascular maturity.

Methods and results

Endothelial knockout of the depalmitoylation enzyme APT-1 (acyl-protein thioesterase 1) in mice impaired recovery from chronic hindlimb ischemia, a model of peripheral artery disease. Endothelial APT-1 deficiency decreased fibronectin processing, disrupted adherens junctions, and inhibited in vitro lumen formation. In an unbiased palmitoylation proteomic screen of endothelial cells from genetically modified mice, R-R

SUBMITTER: Wei X 

PROVIDER: S-EPMC7334103 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature