Broadly neutralizing antibodies potently inhibit cell-to-cell transmission of semen leukocyte-derived SHIV162P3.

ABSTRACT:

Background

HIV-1 sexual transmission occurs mostly through infected semen, which contains both free virions and infected leukocytes. Transmission initiated by infected cells has been shown by several in vitro and in vivo studies and a reduced capacity of broadly neutralizing antibodies (bNAbs) to inhibit cell-to-cell transmission has also been reported. However, due to limitations of available experimental models, there is yet no clarity to which extend bNAbs can prevent transmission mediated by semen leukocytes.

Methods

We developed a novel in vitro assay to measure cell-cell transmission that makes use of splenocytes or CD45⁺ semen leukocytes collected from acutely SHIV_162P3-infected cynomolgus macaques. A panel of 11 bNAbs was used either alone or in

SUBMITTER: Suphaphiphat K 

PROVIDER: S-EPMC7334370 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature