Enzymatic characterization and structure-function relationship of two chitinases, LmChiA and LmChiB, from Listeria monocytogenes.

ABSTRACT: Listeria monocytogenes possesses two chitinases (LmChiA and LmChiB) belonging to glycoside hydrolase family 18 (GH18). In this study, two chitinase genes (lmchiA and lmchiB) from L. monocytogenes 10403S were cloned and their biochemical characteristics were studied. Using colloidal chitin as substrate, both chitinases exhibited maximum catalytic activity at pH 6-7 with optimum temperature at 50 °C. Their activities were stable over broad pH (3-10) and temperature (10-50 °C) ranges. Kinetic analysis using [4NP-(GlcNAc)₂] as substrate indicated that LmChiB had an approximately 4-fold lower K _m and 2-fold higher k _cat than LmChiA, suggesting that the catalytic specificity and efficiency of LmChiB were greater than thos

SUBMITTER: Churklam W 

PROVIDER: S-EPMC7334433 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature