Dynamic measurement of cytosolic pH and [NO₃ ^-] uncovers the role of the vacuolar transporter AtCLCa in cytosolic pH homeostasis.

ABSTRACT: Ion transporters are key players of cellular processes. The mechanistic properties of ion transporters have been well elucidated by biophysical methods. Meanwhile, the understanding of their exact functions in cellular homeostasis is limited by the difficulty of monitoring their activity in vivo. The development of biosensors to track subtle changes in intracellular parameters provides invaluable tools to tackle this challenging issue. AtCLCa (Arabidopsis thaliana Chloride Channel a) is a vacuolar NO₃ ^-/H⁺ exchanger regulating stomata aperture in A thaliana Here, we used a genetically encoded biosensor, ClopHensor, reporting the dynamics of cytosolic anion concentration and pH to monitor the activity of AtCLCa in vivo in Arabidopsis<

SUBMITTER: Demes E 

PROVIDER: S-EPMC7334523 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature