Probing and manipulating embryogenesis via nanoscale thermometry and temperature control.

ABSTRACT: Understanding the coordination of cell-division timing is one of the outstanding questions in the field of developmental biology. One active control parameter of the cell-cycle duration is temperature, as it can accelerate or decelerate the rate of biochemical reactions. However, controlled experiments at the cellular scale are challenging, due to the limited availability of biocompatible temperature sensors, as well as the lack of practical methods to systematically control local temperatures and cellular dynamics. Here, we demonstrate a method to probe and control the cell-division timing in Caenorhabditis elegans embryos using a combination of local laser heating and nanoscale thermometry. Local infrared laser illumination produces a temperature gradient across the embryo, which

SUBMITTER: Choi J 

PROVIDER: S-EPMC7334529 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature