AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions.

ABSTRACT:

Background

mTOR signaling is an essential nutrient and energetic sensing pathway. Here we describe AIMTOR, a sensitive genetically encoded BRET (Bioluminescent Resonance Energy Transfer) biosensor to study mTOR activity in living cells.

Results

As a proof of principle, we show in both cell lines and primary cell cultures that AIMTOR BRET intensities are modified by mTOR activity changes induced by specific inhibitors and activators of mTORC1 including amino acids and insulin. We further engineered several versions of AIMTOR enabling subcellular-specific assessment of mTOR activities. We then used AIMTOR to decipher mTOR signaling in physio-pathological conditions. First, we show that mTORC1 activity increases during muscle cell differentiation and in response to leucine stim

SUBMITTER: Bouquier N 

PROVIDER: S-EPMC7334845 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature