Temporospatial shifts within commercial laboratory mouse gut microbiota impact experimental reproducibility.

ABSTRACT:

Background

Experimental reproducibility in mouse models is impacted by both genetics and environment. The generation of reproducible data is critical for the biomedical enterprise and has become a major concern for the scientific community and funding agencies alike. Among the factors that impact reproducibility in experimental mouse models is the variable composition of the microbiota in mice supplied by different commercial vendors. Less attention has been paid to how the microbiota of mice supplied by a particular vendor might change over time.

Results

In the course of conducting a series of experiments in a mouse model of malaria, we observed a profound and lasting change in the severity of malaria in mice infected with Plasmodium yoelii; while for several years mice obt

SUBMITTER: Mandal RK 

PROVIDER: S-EPMC7334859 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature