Autophagy induction by exogenous polyamines is an artifact of bovine serum amine oxidase activity in culture serum.

ABSTRACT: Polyamines are small polycationic alkylamines involved in many fundamental cellular processes, including proliferation, nucleic acid synthesis, apoptosis, and protection from oxidative damage. It has been proposed that in addition to these functions, elevated levels of polyamines promote longevity in various biological systems, including yeast, Drosophila, and murine models. A series of in vitro mechanistic studies by multiple investigators has led to the conclusion that addition of exogenous spermidine promotes longevity through autophagy induction; however, these experiments were confounded by the use of mammalian cell culture systems supplemented with fetal bovine serum. Using cell viability assays, LC3B immunoblots, and live-cell fluorescence microscopy, we report here th

SUBMITTER: Holbert CE 

PROVIDER: S-EPMC7335804 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature