Unraveling VEALYL Amyloid Formation Using Advanced Vibrational Spectroscopy and Microscopy.

ABSTRACT: Intermediate species are hypothesized to play an important role in the toxicity of amyloid formation, a process associated with many diseases. This process can be monitored with conventional and two-dimensional infrared spectroscopy, vibrational circular dichroism, and optical and electron microscopy. Here, we present how combining these techniques provides insight into the aggregation of the hexapeptide VEALYL (Val-Glu-Ala-Leu-Tyr-Leu), the B-chain residue 12-17 segment of insulin that forms amyloid fibrils (intermolecularly hydrogen-bonded β-sheets) when the pH is lowered below 4. Under such circumstances, the aggregation commences after approximately an hour and continues to develop over a period of weeks. Singular value decompositions of one-dimensional and two-dimensional infrared spe

SUBMITTER: Roeters SJ 

PROVIDER: S-EPMC7335935 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature