Identification of Cartilage Microbial DNA Signatures and Associations With Knee and Hip Osteoarthritis.

ABSTRACT:

Objective

Alterations of the gut microbiota have been implicated in many forms of arthritis, but an examination of cartilage microbial patterns has not been performed. This study was undertaken to characterize the microbial DNA profile of articular cartilage and determine changes associated with osteoarthritis (OA).

Methods

We performed 16S ribosomal RNA gene deep sequencing on eroded and intact cartilage samples from knee OA patients (n = 21 eroded and 21 intact samples) and hip OA patients (n = 34 eroded and 33 intact samples) and cadaver controls (n = 10 knee samples and 10 hip samples). Microbial DNA diversity was assessed, groups were compared, and metagenomic profiles were reconstructed. Confirmation was performed in an independent cohort by clade-specific quantitative

SUBMITTER: Dunn CM 

PROVIDER: S-EPMC7336391 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature