Decoding the evolutionary response to prostate cancer therapy by plasma genome sequencing.

ABSTRACT:

Background

Investigating genome evolution in response to therapy is difficult in human tissue samples. To address this challenge, we develop an unbiased whole-genome plasma DNA sequencing approach that concurrently measures genomic copy number and exome mutations from archival cryostored plasma samples. This approach is applied to study longitudinal blood plasma samples from prostate cancer patients, where longitudinal tissue biopsies from the bone and other metastatic sites have been challenging to collect.

Results

A molecular characterization of archival plasma DNA from 233 patients and genomic profiling of 101 patients identifies clinical correlations of aneuploid plasma DNA profiles with poor survival, increased plasma DNA concentrations, and lower plasma DNA size distri

SUBMITTER: Ramesh N 

PROVIDER: S-EPMC7336456 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature