Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial‑to‑mesenchymal transition through the Wnt/β‑catenin pathway.

ABSTRACT: Mounting evidence indicates that there exists an association between heparanase (HPSE) and several physiological and pathological mechanisms in humans. However, the dynamics of the mechanisms involved in the regulation of HPSE expression in pancreatic cancer (PC) remain unclear. The aim of the present study was to assess the levels of HPSE in PC tissues and cell lines by western blotting and reverse transcription‑quantitative PCR (RT‑qPCR) analysis. Wound healing and Transwell assays were conducted to examine the effects of HPSE on migration and invasion in sh‑NC and sh‑HPSE PC cell lines. In addition, tumor growth was assessed in a mouse xenograft model in vivo. The expression levels of epithelial‑to‑mesenchymal transition (EMT)‑related biomarkers and the involvement of the Wnt/β‑catenin

SUBMITTER: Wang C 

PROVIDER: S-EPMC7336506 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature